Journal:Accelerated solvent extraction of terpenes in cannabis coupled with various injection techniques for GC-MS analysis
| Full article title | Accelerated solvent extraction of terpenes in cannabis coupled with various injection techniques for GC-MS analysis |
|---|---|
| Journal | Frontiers in Chemistry |
| Author(s) | Myers, Colton; Herrington, Jason S.; Hamrah, Paul; Anderson, Kelsey |
| Author affiliation(s) | Restek Corporation, Verity Analytics |
| Primary contact | colton dot myers at restek dot com |
| Year published | 2021 |
| Volume and issue | 9 |
| Article # | 619770 |
| DOI | 10.3389/fchem.2021.619770 |
| ISSN | 2296-2646 |
| Distribution license | Creative Commons Attribution 4.0 International |
| Website | https://www.frontiersin.org/articles/10.3389/fchem.2021.619770/full |
| Download | https://www.frontiersin.org/articles/10.3389/fchem.2021.619770/pdf (PDF) |
 |
This article should be considered a work in progress and incomplete. Consider this article incomplete until this notice is removed. |
Abstract
The cannabis market is expanding exponentially in the United States. As state-wide legalization efforts increase, so also do demands for analytical testing methodologies. One of the main tests conducted on cannabis products is the analysis for terpenes. This research focused on implementation of accelerated solvent extraction (ASE), utilizing surrogate matrix matching, and evaluation of traditional vs. more modern sample introduction techniques for analyzing terpenes via gas chromatography–mass spectrometry (GC-MS). Introduction techniques included headspace syringe (HS syringe), HS-solid-phase microextraction Arrow (HS-SPME Arrow), direct immersion-SPME Arrow (DI-SPME Arrow), and liquid injection syringe (LI syringe). The LI syringe approach was deemed the most straightforward and robust method, with terpene working ranges of 0.04–5.12 μg/mL; r2 values of 0.988–0.996 (0.993 average); limit of quantitation values of 0.017–0.129 μg/mL (0.047 average); analytical precisions of 2.58–9.64% RSD (1.56 average); overall ASE-LI-syringe-GC-MS method precisions of 1.73–14.6% RSD (4.97 average); and % recoveries of 84.6–98.9% (90.2 average) for the 23 terpenes of interest. Sample workflows and results are discussed, with an evaluation of the advantages/limitations of each approach and opportunities for future work.
Keywords: accelerated solvent extraction (ASE), terpenes, solid-phase microextraction (SPME), solid-phase microextraction Arrow (SPME Arrow), gas chromatography–mass spectrometry (GC-MS)
Introduction
References
Notes
This presentation is faithful to the original, with only a few minor changes to presentation. Some grammar and punctuation was cleaned up to improve readability. In some cases important information was missing from the references, and that information was added. The original article lists references in alphabetical order; this wiki organizes them by order of appearance, by design.
