Terpene

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Many terpenes are derived commercially from conifer resins, such as those made by this pine.

Terpenes (/ˈtɜːrpn/) are a class of natural products consisting of compounds with the formula (C5H8)n. Comprising more than 30,000 compounds, these unsaturated hydrocarbons are produced predominantly by plants, particularly conifers.[1][2] Terpenes are further classified by the number of carbons: monoterpenes (C10), sesquiterpenes (C15), diterpenes (C20), etc. A well known monoterpene is alpha-pinene, a major component of turpentine.

History and terminology

The term "terpene" was coined in 1866 by the German chemist August Kekulé.[3] The name "terpene" is a shortened form of "terpentine", an obsolete spelling of "turpentine".[4]

Although sometimes used interchangeably with "terpenes", terpenoids (or isoprenoids) are modified terpenes that contain additional functional groups, usually oxygen-containing.[5] Terpenoids are terpenes that have been modified with (usually oxygen-containing) functional groups. The terms terpenes and terpenoids are used interchangeably by some. Both have strong and often pleasant odors, which may protect their hosts or attract pollinators. The inventory of terpenes and terpenoids is estimated at 55,000 chemical entities.[6]

The 1939 Nobel Prize in Chemistry was awarded to Leopold Ružička "for his work on polymethylenes and higher terpenes",[7][8] "including the first chemical synthesis of male sex hormones."[9]

Biological function

Terpenes are also major biosynthetic building blocks. Steroids, for example, are derivatives of the triterpene squalene. Terpenes and terpenoids are also the primary constituents of the essential oils of many types of plants and flowers.[10] In plants, terpenes and terpenoids are important mediators of ecological interactions. For example, they play a role in plant defense against herbivory, disease resistance, attraction of mutualists such as pollinators, as well as potentially plant-plant communication.[11][12] They appear to play roles as antifeedants[2] Other functions of terpenoids include "cell growth modulation and plant elongation, light harvestation and photoprotection, and membrane permeability and fluidity control.[13]

Higher amounts of terpenes are released by trees in warmer weather, where they may function as a natural mechanism of cloud seeding. The clouds reflect sunlight, allowing the forest temperature to regulate.[14]

Some insects use some terpenes as a form of defense. For example, termites of the subfamily Nasutitermitinae to ward off predatory insects, through the use of a specialized mechanism called a fontanellar gun, which ejects a resinous mixture of terpenes.[15]

Applications

Structure of natural rubber, exhibiting the characteristic methyl group on the alkene group.

The one terpene that has major applications is natural rubber (i.e. polyisoprene). The possibility that other terpenes could be used as precursors to produce synthetic polymers has been investigated as an alternative to the use of petroleum-based feedstocks. However, few of these applications have been commercialized.[16] Many other terpenes, however, have smaller scale commercial and industrial applications. For example, turpentine, a mixture of terpenes (e.g. pinene), obtained from the distillation of pine tree resin, is used an organic solvent and as a chemical feedstock (mainly for the production of other terpenoids).[4] Rosin, another by-product of conifer tree resin, is widely used as an ingredient in a variety of industrial products, such as inks, varnishes and adhesives. Terpenes are widely used as fragrances and flavors in consumer products such as perfumes, cosmetics and cleaning products, as well as food and drink products. For example, the aroma and flavor of hops comes, in part, from sesquiterpenes (mainly α-humulene and β-caryophyllene), which affect beer quality.[17]They are also components of some traditional medicines, such as aromatherapy. Some form hydroperoxides that are valued as catalysts in the production of polymers.

Reflecting their defensive role, terpenes are used as active ingredients of natural pesticides in agriculture.[18]

Physical and chemical properties

Terpenes are colorless, although impure samples are often yellow. Boiling points scale with molecular size: terpenes, sesquiterpenes, and diterpenes respectively at 110, 160, and 220 °C. Being highly non-polar, they are insoluble in water. Being hydrocarbons, they are highly flammable and have low specific gravity (float on water).

Terpenoids (mono-, sesqui-, di-, etc.) have similar physical properties but tend to be more polar and hence slightly more soluble in water and somewhat less volatile than their terpene analogues. Highly polar derivative of terpenoids are the glycosides, which are linked to sugars. They are water-soluble solids. They are tactilely light oils considerably less viscous than familiar vegetable oils like corn oil (28 cP), with viscosity ranging from 1 cP (ala water) to 6 cP. Like other hydrocarbons, they are highly flammable. Terpenes are local irritants and can cause gastrointestinal disturbances if ingested.

Biosynthesis

Biosynthetic conversion of geranylpyrophosphate to the terpenes α-pinene and β-pinene and to the terpinoid α-terpineol.[2]

Conceptually derived from isoprenes, the structures and formulas of terpenes follow the biogenetic isoprene rule or the C5 rule, as described in 1953 by Leopold Ružička[19] and colleagues.[20] The isoprene units are provided from isoprenyl pyrophosphate (aka dimethylallyl pyrophosphate) and isopentenyl pyrophosphate, which exist in equilibrium. This pair of building blocks are produced by two distinct metabolic pathways: the mevalonic acid pathway (MVA) and the MEP/DOXP pathway. The MVA and MEP/DOXP are mutually exclusive in most organisms.[citation needed]

Organism Pathways
Bacteria MVA or MEP
Archaea MVA
Green Algae MEP
Plants MVA and MEP
Animals MVA
Fungi MVA

Mevalonic acid pathway

Most organisms produce terpenes through the HMG-CoA reductase pathway, known as the Mevalonate pathway, named for the intermediates mevalonic acid. This pathway begins with acetyl CoA.

MEP/DOXP pathway

The 2-C-methyl-D-erythritol 4-phosphate/1-deoxy-D-xylulose 5-phosphate pathway (MEP/DOXP pathway), also known as non-mevalonate pathway or mevalonic acid-independent pathway, starts with pyruvate as the carbon source.

Pyruvate and glyceraldehyde 3-phosphate are converted by DOXP synthase (Dxs) to 1-deoxy-D-xylulose 5-phosphate, and by DOXP reductase (Dxr, IspC) to 2-C-methyl-D-erythritol 4-phosphate (MEP). The subsequent three reaction steps catalyzed by 4-diphosphocytidyl-2-C-methyl-D-erythritol synthase (YgbP, IspD), 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (YchB, IspE), and 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (YgbB, IspF) mediate the formation of 2-C-methyl-D-erythritol 2,4-cyclopyrophosphate (MEcPP). Finally, MEcPP is converted to (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate (HMB-PP) by HMB-PP synthase (GcpE, IspG), and HMB-PP is converted to isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP) by HMB-PP reductase (LytB, IspH).

IPP and DMAPP are the end-products in either pathway, and are the precursors of isoprene, monoterpenoids (10-carbon), diterpenoids (20-carbon), carotenoids (40-carbon), chlorophylls, and plastoquinone-9 (45-carbon). Synthesis of all higher terpenoids proceeds via formation of geranyl pyrophosphate (GPP), farnesyl pyrophosphate (FPP), and geranylgeranyl pyrophosphate (GGPP).

Geranyl pyrophosphate phase and beyond

Isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP) condense to produce geranyl pyrophosphate, precursor to all terpenes and terpenoids.

In both MVA and MEP pathways, IPP is isomerized to DMAPP by the enzyme isopentenyl pyrophosphate isomerase. IPP and DMAPP condense to give geranyl pyrophosphate, the precursor to monoterpenes and monoterpenoids.

Geranyl pyrophosphate is also converted to farnesyl pyrophosphate and geranylgeranyl pyrophosphate, respectively C15 and C20 precursors to sesquiterpenes and diterpenes (as well as sesequiterpenoids and diterpenoids).[2] Biosynthesis is mediated by terpene synthase.[21][22]

Terpenes to terpenoids

The genomes of 17 plant species contain genes that encode terpenoid synthase enzymes imparting terpenes with their basic structure, and cytochrome P450s that modify this basic structure.[2][23]

Structure

Terpenes can be visualized as the result of linking isoprene (C5H8) units "head to tail" to form chains and rings.[24] A few terpenes are linked “tail to tail”, and larger branched terpenes may be linked “tail to mid”.

Formula

Strictly speaking all monoterpenes have the same chemical formula C10H16. Similarly all sequiterpenes and diterpenes are respectively C15H24 and C20H32. The structural diversity of mono-, sesqui-, and diterpenes is a consequence of isomerism.

Chirality

Terpenes and terpenoids are usually chiral. Chiral compounds can exist as non-superimposable mirror images, which exhibit distinct physical properties such as odor or toxicity.

Unsaturation

Most terpenes and terpenoids feature C=C groups, i.e. they exhibit unsaturation. Since they carry no functional groups aside from their unsaturation, terpenes are structurally distinctive. The unsaturation is associated with di- and trisubstituted alkenes. Di- and trisubstituted alkenes resist polymerization (low ceiling temperatures) but are susceptible to acid-induced carbocation formation.

Classification

Terpenes may be classified by the number of isoprene units in the molecule; a prefix in the name indicates the number of isoprene pairs needed to assemble the molecule. Commonly, terpenes contain 2, 3, 4 or 6 isoprene units; the tetraterpenes (8 isoprene units) form a separate class of compounds called carotenoids; the others are rare.

Second- or third-instar caterpillars of Papilio glaucus emit terpenes from their osmeterium.

Industrial syntheses

While terpenes and terpenoids occur widely, their extraction from natural sources is often problematic. Consequently, they are produced by chemical synthesis, usually from petrochemicals. In one route, acetone and acetylene are condensed to give 2-Methylbut-3-yn-2-ol, which is extended with acetoacetic ester to give geranyl alcohol. Others are prepared from those terpenes and terpenoids that are readily isolated in quantity, say from the paper and tall oil industries. For example, α-pinene, which is readily obtainable from natural sources, is converted to citronellal and camphor. Citronellal is also converted to rose oxide and menthol.[1]

Summary of an industrial route to geranyl alcohol from simple reagents.

References

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  2. ^ a b c d e Davis, Edward M.; Croteau, Rodney (2000). "Cyclization enzymes in the biosynthesis of monoterpenes, sesquiterpenes, and diterpenes". Biosynthesis. Topics in Current Chemistry. 209. pp. 53–95. doi:10.1007/3-540-48146-X_2. ISBN 978-3-540-66573-1.CS1 maint: uses authors parameter (link)
  3. ^ Kekulé coined the term "terpene" to denote all hydrocarbons having the empirical formula C10H16, of which camphene was one. Previously, many hydrocarbons having the empirical formula C10H16 had been called "camphene", but many other hydrocarbons of the same composition had had different names. Hence Kekulé coined the term "terpene" in order to reduce the confusion. See:
    • Kekulé, August (1866). Lehrbuch der organischen Chemie [Textbook of Organic Chemistry] (in German). vol. 2. Erlangen, (Germany): Ferdinand Enke. pp. 464–465. |volume= has extra text (help) From pp. 464–465: "Mit dem Namen Terpene bezeichnen wir … unter verschiedenen Namen aufgeführt werden." (By the name "terpene" we designate in general the hydrocarbons composed according to the [empirical] formula C10H16 (see §. 1540). Many chemists subsume the hydrocarbons of the formula C10H16 under the general name "camphene". That name seems unsuitable, because a specific substance of this group has been designated as "camphene". In general, a great confusion prevails in the designation of substances belonging here [i.e., to the terpene group]. Many, obviously different hydrocarbons have not been distinguished for a long time and have been assigned the same names, while on the other hand, probably identical substances from different sources have often been specified by different names.)
    • Dev, Sukh (1989). "Chapter 8. Isoprenoids: 8.1. Terpenoids.". In Rowe, John W. (ed.). Natural Products of Woody Plants: Chemicals Extraneous to the Lignocellulosic Cell Wall. Berlin and Heidelberg, Germany: Springer-Verlag. pp. 691–807. ; see p. 691.
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Notes

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