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Table 3. Complete composition and extraction yield (% w/w dry weight) of the essential oil obtained from the dried inflorescences and floral bracts of 2020 hemp samples.
 
1 LRI = Linear retention index on a HP 5-MS capillary column; 2 Not detected; 3 Compounds accounting for at least 1.000% of the dissimilarity rate (according to the SIMPER test; see Table 4) are evidenced in bold. For these compounds, for all chemical classes, and for the extraction yield, different superscript uppercase letters (AG) indicate statistically significant differences between each variety; superscript lowercase letters (a,b) indicate statistically significant differences among the cultivar withdrawn on different years (see Table 2 for 2019 samples). The statistical significance of the relative abundances was established by the Tukey’s post-hoc test, with p ≤ 0.05.
Relative abundance (%) ± SD
Compounds LRI1 Monoecious Dioecious
Carmaleonte Codimono Felina 32 Futura 75 Uso-31 Bernabeo Carmagnola CS Eletta Campana Fibranova Fibrante
heptanal 901 -2 - - - - 0.3 ± 0.06 0.1 ± 0.10 0.1 ± 0.04 0.1 ± 0.07 - -
α-pinene3 933 2.7 ± 0.50AB;a 2.0 ± 0.74ABC;a 1.1 ± 0.40CD;a 1.1 ± 0.11CD;a 2.0 ± 0.26ABC;a 0.5 ± 0.06D;a 3.0 ± 0.04A;a 2.1 ± 0.79ABC;a 1.7 ± 0.01BC;a 1.3 ± 0.39CD;a 1.2 ± 0.06CD;a
β-pinene 977 0.5 ± 0.06 0.5 ± 0.17 0.3 ± 0.07 0.2 ± 0.06 0.7 ± 0.18 0.1 ± 0.01 0.8 ± 0.09 0.7 ± 0.26 0.5 ± 0.02 0.3 ± 0.12 0.4 ± 0.03
myrcene3 991 0.8 ± 0.09CD;a 0.8 ± 0.33CD;a 0.2 ± 0.02D;a 0.4 ± 0.09D;a 0.6 ± 0.12D;a 0.4 ± 0.05D;a 2.6 ± 0.16A;a 1.3 ± 0.49BC;a 0.6 ± 0.01D;a 0.3 ± 0.10D;a 1.8 ± 0.17B;a
δ-3-carene 1011 0.1 ± 0.05 - - - - - - - 0.1 ± 0.01 0.3 ± 0.09 -
α-terpinene 1017 - - - - - - - 0.1 ± 0.06 - - -
limonene 1029 0.1 ± 0.01 0.1 ± 0.08 - - 0.2 ± 0.01 0.1 ± 0.03 0.6 ± 0.10 0.7 ± 0.16 0.4 ± 0.01 0.1 ± 0.08 0.8 ± 0.08
1,8-cineole 1031 - 0.2 ± 0.05 - - - 0.2 ± 0.05 0.2 ± 0.09 0.2 ± 0.06 0.1 ± 0.02 0.2 ± 0.06 0.1 ± 0.06
(E)-β-ocimene 1047 0.1 ± 0.01 0.4 ± 0.13 - 0.1 ± 0.03 0.4 ± 0.02 - 0.3 ± 0.02 0.1 ± 0.06 0.2 ± 0.01 0.3 ± 0.07 -
γ-terpinene 1058 - - - - - - - 0.1 ± 0.07 - - -
terpinolene 1089 - 0.4 ± 0.14 0.2 ± 0.04 0.1 ± 0.09 0.1 ± 0.02 0.1 ± 0.01 0.3 ± 0.03 0.8 ± 0.19 0.3 ± 0.01 - 0.5 ± 0.03
nonanal 1105 0.1 ± 0.07 - - - 0.1 ± 0.06 0.2 ± 0.04 - - - - -
linalool 1101 - 0.1 ± 0.06 - - - - 0.2 ± 0.01 0.2 ± 0.04 0.1 ± 0.03 - -
fenchol 1114 - - - - - - 0.2 ± 0.02 0.4 ± 0.07 0.2 ± 0.05 - 0.3 ± 0.00
cis-p-menth-2-en-1-ol 1122 1114 - - - - - - 0.2 ± 0.02 0.2 ± 0.03 0.2 ± 0.02 - 0.2 ± 0.01
trans-pinocarveol 1139 0.3 ± 0.03 - - - 0.1 ± 0.01 - - - - - -
trans-verbenol 1145 - - - - 0.2 ± 0.01 - - - - - -
ipsdienol 1147
β-pinene oxide 1156 - - - - - - 0.3 ± 0.03 - - - 0.1 ± 0.01
borneol 1165 0.2 ± 0.02 0.1 ± 0.05 - - - 0.1 ± 0.00 0.2 ± 0.02 0.2 ± 0.04 0.2 ± 0.00 0.1 ± 0.06 0.2 ± 0.01
lavandulol 1170 - 0.4 ± 0.09 0.3 ± 0.06 0.2 ± 0.05 0.2 ± 0.05 0.4 ± 0.06 0.3 ± 0.01 0.6 ± 0.03 0.3 ± 0.01 - 0.6 ± 0.04
4-terpineol 1177 - - - - - - - - - 0.2 ± 0.03 -
p-cymen-8-ol 1185 - - - - - - - - - - -
myrtenal 1194 - - - - - - - - - - -
α-terpineol 1191 - 0.2 ± 0.03 - - - 0.2 ± 0.04 0.3 ± 0.01 0.5 ± 0.04 - 0.2 ± 0.03 0.5 ± 0.02
eugenol 1357 0.2 ± 0.06 0.1 ± 0.07 - 0.1 ± 0.03 0.1 ± 0.01 - - - - 0.2 ± 0.07 -
methyl eugenol 1405 - - - - 0.1 ± 0.02 - - - - - -
α-ylangene 1374 0.2 ± 0.01 0.1 ± 0.07 - 0.1 ± 0.01 - - 0.1 ± 0.04 0.1 ± 0.02 0.3 ± 0.01 0.1 ± 0.07 -
isocaryophyllene 1407 0.4 ± 0.03 0.4 ± 0.00 0.3 ± 0.09 0.4 ± 0.03 0.2 ± 0.01 0.3 ± 0.11 0.2 ± 0.04 0.3 ± 0.06 0.3 ± 0.01 0.5 ± 0.12 0.3 ± 0.01
cis-α-bergamotene 1416 - 0.1 ± 0.01 0.4 ± 0.14 0.1 ± 0.09 - - - - - - -
β-caryophyllene3 1419 15.3 ± 1.44AB;a 16.2 ± 1.46AB;a 11.5 ± 3.55ABC;a 13.8 ± 0.59ABC;a 8.7 ± 0.77C;a 10.4 ± 2.95BC;a 14.4 ± 0.89ABC;a 15.4 ± 2.45AB;a 11.6 ± 0.09ABC;a 16.8 ± 3.82A;a 16.2 ± 0.8AB;a
trans-α-bergamotene 1436 0.3 ± 0.00 1.1 ± 0.04 1.6 ± 0.42 1.5 ± 0.16 1.0 ± 0.06 0.3 ± 0.06 0.1 ± 0.01 0.1 ± 0.08 0.3 ± 0.01 0.9 ± 0.2 -
α-guaiene 1439 - - - - - 0.6 ± 0.14 - - - - -
aromadendrene 1142 - - - - - - - - - 0.3 ± 0.04 -
guaia-6,9-diene 1443 0.2 ± 0.01 - - - - - - - 0.1 ± 0.05 0.1 ± 0.05 -
isogermacrene D 1451 0.3 ± 0.03 - - - - - - - 0.2 ± 0.01 - -
α-humulene3 1453
aristolene 1452
(E)-β-farnesene 1458
alloaromadendrene 1460
4,5-di-epi-aristolochene 1468
γ-gurjunene 1469
β-chamigrene 1476
γ-muurolene 1477
α-amorphene 1482
γ-selinene 1483
β-selinene3 1486
δ-selinene 1491
valencene 1493
α-selinene 1495
eremophilene 1499
α-bulnesene 1505
epizonarene 1501
β-bisabolene 1509
(E,E)-α-farnesene 1509
β-curcumene 1513
trans-γ-cadinene 1513
sesquicineole 1516
7-epi-α-selinene 1517
(Z)-γ-bisabolene 1519
β-cadinene 1519
trans-calamenene 1524
δ-cadinene 1524
selina-3,7(11)-diene3 1530
(E)-γ-bisabolene 1531
α-calacorene 1543
cis-sesquisabinene hydrate 1545
elemol 1550
guaia-3,9-diene 1556
(E)-nerolidol 1564
palustrol 1568
caryophyllene alcohol 1570
spathulenol 1577
trans-sesquisabinene hydrate 1581
caryophyllene oxide3 1582
globulol 1583
trans-(Z)-α-bisabolene epoxide 1586
isoaromadendrene epoxide 1589
epi-globulol 1590
viridiflorol 1592
guaiol 1596
humulene oxide I3 1608
cedrenol 1610
humulane-1-6-dien-3-ol 1613
selin-6-en-4-ol3 1618
13-nor-valenc-1(10)-en-11-one 1629
1-epi-cubenol 1627
γ-eudesmol 1631
caryophylla-4(14),8(15)-dien-5-ol (unidentified isomer 1)3 1633
caryophylla-4(14),8(15)-dien-5-ol (unidentified isomer 2)3 1633
T-cadinol 1641
cubenol 1641
β-eudesmol 1649
α-eudesmol 1653
neointermedeol 1655
bulnesol 1668
14-hydroxy-9-epi-(E)-caryophyllene 1670
ylangenal 1675
aromadendrene epoxide II 1680
α-bisabolol3 1685
cedr-8-en-13-ol 1688
juniper camphor 1694
(Z)-α-trans-bergamotol 1700
α-phellandrene dimer 1801
nootkatone 1803
(E,E)-farnesyl acetate 1843
hexahydrofarnesylacetone3 1845
1-hexadecanol 1877
(E,E)-farnesyl acetone 1918
m-camphorene 1952
p-camphorene 1986
ethyl hexadecanoate 2000
phytol3 2112
cannabidiol3 2369
cannabichromene 2373
Δ9-tetrahydrocannabinol 2468
Total identified (%)
Monoterpene hydrocarbons
Oxygenated monoterpenes
Sesquiterpene hydrocarbons
Oxygenated sesquiterpenes
Diterpene hydrocarbons
Oxygenated diterpenes
Phenylpropanoids
Cannabinoids
Apocarotenoids
Other non-terpene derivatives
Extraction yield (% w/w)