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"High-throughput methods to identify male Cannabis sativa using various genotyping methods"

"Cannabis sativa is a primarily dioecious angiosperm that exhibits sexual developmental plasticity. Developmental genes for staminate male flowers have yet to be elucidated; however, there are regions of male-associated DNA from Cannabis (MADC) that correlate with the formation of pollen-producing staminate flowers. MADC2 is an example of a polymerase chain reaction-based (PCR-based) genetic marker that has been shown to produce a 390-bp amplicon that correlates with the expression of male phenotypes. We demonstrate applications of a cost-effective high-throughput male genotyping assay and other genotyping applications of male identification in Cannabis sativa ... (Full article...)

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