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With an increasing appreciation for the unique pharmacological properties associated with distinct, individual cannabinoids of Cannabis sativa, there is demand for accurate and reliable quantification for a growing number of them. Although recent methods are based on highly selective chromatography–mass spectrometry technology, most are limited to a few cannabinoids, while relying on unnecessarily sophisticated and expensive ultra-high-performance liquid chromatography and tandem mass spectrometry. Here we report an optimized, simple extraction method followed by a reliable and simple high-performance liquid chromatography (HPLC) method for separation. The detection is performed using a time-of-flight mass spectrometer that is available in most natural products research laboratories ... (Full article...)
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