Journal:Development of a gas-chromatographic method for simultaneous determination of cannabinoids and terpenes in hemp
Full article title | Development of a gas-chromatographic method for simultaneous determination of cannabinoids and terpenes in hemp |
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Journal | Molecules |
Author(s) | Zekič, Jure; Križman, Mitja |
Author affiliation(s) | National Institute of Chemistry - Ljubljana, University of Ljubljana |
Primary contact | Email: mitja dot krizman at ki dot si |
Year published | 2020 |
Volume and issue | 25(24) |
Article # | 5872 |
DOI | 10.3390/molecules25245872 |
ISSN | 1420-3049 |
Distribution license | Creative Commons Attribution 4.0 International |
Website | https://www.mdpi.com/1420-3049/25/24/5872/htm |
Download | https://www.mdpi.com/1420-3049/25/24/5872/pdf (PDF) |
This article should be considered a work in progress and incomplete. Consider this article incomplete until this notice is removed. |
Abstract
An original gas-chromatographic method has been developed for simultaneous determination of major terpenes and cannabinoids in plant samples and their extracts. The main issues to be addressed were not only the large differences in polarity and volatility between both groups of analytes, but also the need for an exhaustive decarboxylation of cannabinoid acidic forms. Sample preparation was minimized by avoiding any analyte derivatization. Acetone was found to be the most appropriate extraction solvent. Successful chromatographic separation was achieved by using a medium-polarity column. Limits of detection ranged from 120 to 260 ng/mL for terpenes and from 660 to 860 ng/mL for cannabinoids. Parallel testing proved the results for cannabinoids are comparable to those obtained from established high-performance liquid chromatography (HPLC) methods. Despite very large differences in concentrations between both analyte groups, a linear range between 1 and 100 µg/mL for terpenes and between 10 and 1500 µg/mL for cannabinoids was determined.
Keywords: cannabinoids, terpenes, cannabis, hemp, gas chromatography, capillary column
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This presentation is faithful to the original, with only a few minor changes to presentation. Some grammar and punctuation was cleaned up to improve readability. In some cases important information was missing from the references, and that information was added.